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Haplotype divergence in Beta vulgaris and microsynteny with sequenced plant genomes.

Identifieur interne : 003604 ( Main/Exploration ); précédent : 003603; suivant : 003605

Haplotype divergence in Beta vulgaris and microsynteny with sequenced plant genomes.

Auteurs : Juliane C. Dohm [Allemagne] ; Cornelia Lange ; Richard Reinhardt ; Heinz Himmelbauer

Source :

RBID : pubmed:18764921

Descripteurs français

English descriptors

Abstract

We characterized two overlapping sugar beet (Beta vulgaris) bacterial artificial chromosome (BAC) clones representing different haplotypes. A total of 254 kbp of the genomic sequence was determined, of which the two BACs share 92 kbp. Eleven of 15 genes discovered in the sequenced interval locate to the overlap region. The haplotypes differ in exons by 1% (nucleotide level) and in non-coding regions by 9% (6% mismatches, 3% gaps; alignable regions only). Large indels or high sequence divergence comprised 11% of either sequence. Of such indels, 68 and 45%, respectively, could be attributed to haplotype-specific integration of transposable elements. We identified novel repeat candidates by comparing the two BAC sequences to a set of genomic sugar beet sequences. Synteny was found with Arabidopsis chromosome 1 (At1), At2 and At4, Medicago chromosome 7, Vitis chromosome 15 and paralogous regions on poplar chromosomes II and XIV.

DOI: 10.1111/j.1365-313X.2008.03665.x
PubMed: 18764921


Affiliations:


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Le document en format XML

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<name sortKey="Dohm, Juliane C" sort="Dohm, Juliane C" uniqKey="Dohm J" first="Juliane C" last="Dohm">Juliane C. Dohm</name>
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<nlm:affiliation>Max Planck Institute for Molecular Genetics, Ihnestrasse 63-73, Berlin, Germany.</nlm:affiliation>
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<name sortKey="Lange, Cornelia" sort="Lange, Cornelia" uniqKey="Lange C" first="Cornelia" last="Lange">Cornelia Lange</name>
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<name sortKey="Reinhardt, Richard" sort="Reinhardt, Richard" uniqKey="Reinhardt R" first="Richard" last="Reinhardt">Richard Reinhardt</name>
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<name sortKey="Himmelbauer, Heinz" sort="Himmelbauer, Heinz" uniqKey="Himmelbauer H" first="Heinz" last="Himmelbauer">Heinz Himmelbauer</name>
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<term>Base Composition (MeSH)</term>
<term>Beta vulgaris (genetics)</term>
<term>Chromosomes, Artificial, Bacterial (MeSH)</term>
<term>Chromosomes, Plant (MeSH)</term>
<term>DNA, Plant (genetics)</term>
<term>Gene Library (MeSH)</term>
<term>Gene Order (MeSH)</term>
<term>Genes, Plant (MeSH)</term>
<term>Genome, Plant (MeSH)</term>
<term>Genotype (MeSH)</term>
<term>Haplotypes (MeSH)</term>
<term>Polymorphism, Genetic (MeSH)</term>
<term>Sequence Alignment (MeSH)</term>
<term>Sequence Analysis, DNA (MeSH)</term>
<term>Synteny (MeSH)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr">
<term>ADN des plantes (génétique)</term>
<term>Alignement de séquences (MeSH)</term>
<term>Analyse de séquence d'ADN (MeSH)</term>
<term>Banque de gènes (MeSH)</term>
<term>Beta vulgaris (génétique)</term>
<term>Chromosomes artificiels de bactérie (MeSH)</term>
<term>Chromosomes de plante (MeSH)</term>
<term>Composition en bases nucléiques (MeSH)</term>
<term>Gènes de plante (MeSH)</term>
<term>Génome végétal (MeSH)</term>
<term>Génotype (MeSH)</term>
<term>Haplotypes (MeSH)</term>
<term>Ordre des gènes (MeSH)</term>
<term>Polymorphisme génétique (MeSH)</term>
<term>Synténie (MeSH)</term>
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<term>DNA, Plant</term>
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<keywords scheme="MESH" qualifier="genetics" xml:lang="en">
<term>Beta vulgaris</term>
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<term>ADN des plantes</term>
<term>Beta vulgaris</term>
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<term>Gene Order</term>
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<term>Genome, Plant</term>
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<term>Haplotypes</term>
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<term>Sequence Analysis, DNA</term>
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<term>Banque de gènes</term>
<term>Chromosomes artificiels de bactérie</term>
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<term>Composition en bases nucléiques</term>
<term>Gènes de plante</term>
<term>Génome végétal</term>
<term>Génotype</term>
<term>Haplotypes</term>
<term>Ordre des gènes</term>
<term>Polymorphisme génétique</term>
<term>Synténie</term>
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<front>
<div type="abstract" xml:lang="en">We characterized two overlapping sugar beet (Beta vulgaris) bacterial artificial chromosome (BAC) clones representing different haplotypes. A total of 254 kbp of the genomic sequence was determined, of which the two BACs share 92 kbp. Eleven of 15 genes discovered in the sequenced interval locate to the overlap region. The haplotypes differ in exons by 1% (nucleotide level) and in non-coding regions by 9% (6% mismatches, 3% gaps; alignable regions only). Large indels or high sequence divergence comprised 11% of either sequence. Of such indels, 68 and 45%, respectively, could be attributed to haplotype-specific integration of transposable elements. We identified novel repeat candidates by comparing the two BAC sequences to a set of genomic sugar beet sequences. Synteny was found with Arabidopsis chromosome 1 (At1), At2 and At4, Medicago chromosome 7, Vitis chromosome 15 and paralogous regions on poplar chromosomes II and XIV.</div>
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